Confocal Microscopy (CFM) | Transmission
Transmission experiments in confocal microscopy sometimes also require filtering and shaping of the optical beam, and hence free-beam access instead of purely fiber-based can have quite some advantages in terms of flexibility.
This instrument literally consists of a ‚conventional‘ attoCFM I, which has a free-beam based channel with any of our apochromatic objectives above the sample, and a fiber-based channel from below just like in the attoCFM III.
So, despite fitting into any of our toploading cryostats, which usually features only 1 top and no side or bottom windows, this setup allows for at least either the excitation or the detection to be free-beam. This provides the user with all the flexibility of the external optics head just like in case of the attoCFM I for one channel (plus potential reflection measurements).
At the same time, there are 3 degrees of freedom for the sample (xy translation under the fixed LT-APO objective as well as focussing), and another 3 degrees of freedom in xyz to position the fiber-based objective underneath the sample. All positioners are usually equipped with a /RES encoder for closed loop control, and there is an additional scanner for the sample in xy direction.
- ASC400 scan controller
- LDM600 laser source
- LDM600 2nd detector
- attoCFM I external optics head incl. optional filters
- Optical top window
- Free-beam based cryogenic LT-APO objective
- Sample with xyz positioners and optional xy scanner
- Fiber-based cryogenic ashperic objective
- xyz positioner for fiber-based objective
- vacuum feedthrough for single-mode fiber